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The target sequence GTGTCAGGAGGTGTTTGGAAAG (SEQ ID NO: 2) was inserted into pSUPER vector (Oligoengine, Seattle WA) which drives endogenous production of shRNA under the HI promoter

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Type 2 diabetes was associated with roughly twofold higher NNMT expression in both depots, and plasma 1-MNA correlated with both tissue NNMT expression and the degree of insulin resistance